Succinatimonas hippei ET63

Proteobacteria Gammaproteobacteria Aeromonadales Succinivibrionaceae

Genome size: 2 397 496 bp

Genomic GC% content: 40.20 %

Scanning electron microscopy (20 000x magnification)

TOP 15 enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Fructose-bisphosphate aldolase class 2 (EC 4.1.2.13) 391 Glycolysis
Triosephosphate isomerase (EC 5.3.1.1) 257 Glycolysis
Glyceraldehyde-3-phosphate dehydrogenase A (EC 1.2.1.12) 3970 Glycolysis
Phosphoglycerate kinase (EC 2.7.2.3) 562 Glycolysis
Phosphoenolpyruvate carboxykinase (ATP) (EC 4.1.1.49) 4607 Glycolysis
Transketolase 1 (EC 2.2.1.1) 463 Pentose phosphate pathway
Formate acetyltransferase (EC 2.3.1.54) 1814 Pyruvate metabolism
Fumarate reductase flavoprotein subunit (EC=1.3.5.4) 662 Anaerobic respiration
Malate dehydrogenase (EC 1.1.1.37) 476 Citrate cycle (TCA cycle)
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) 467 Citrate cycle (TCA cycle)
Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) 337 Urea cycle
Argininosuccinate synthase (EC 6.3.4.5) 416 Urea cycle
Phosphoribosylformylglycinamidine synthase (EC 6.3.5.3) 341 Purine metabolism
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6) 309 Purine metabolism
Erythritol/L-threitol dehydrogenase (EC 1.1.1.-) 367

MS raw data: ET63

culture medium: Schaedler agar + 20mM succinate

16S rRNA sequence:

TAGGTGAGGTAAAGGCTCACCTAGGCGACGATCTCTAGCCGGTCTGAGAGGATGACCGGCCACATCGGGACTGAGACACGGCCCGGACTCCTACGGGAGGCAGCAGTAGGGAATATTGCACAATGGGGGGAACCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTTCGGGTTGTAAAGCACTTTCAGCGGGGAGGAAATTAAGTTAGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCAGGCGGGCTGATAAGTAAGGTGTGAAATACCGGGGCTCAACCTCGGGGCTGCATTTTAAACTGTCAGTCTAGAGTATTGCAGAGGGAGACGGAATTCCGGGTGTAGCGGTGAAATGCGTAGATATCCGGAAGAACACCGGAGGCGAAGGCGGTCTTCTGGGCAAATACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGATTAGGAGATTGAGCAGAAGCTTGGTTTTCGGAGCAAACGCGCTAAATCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGACCTTGACATCTGAGGAATGCCTGAGAGATTAGGCAGTGCCTTCGGGAGCCTCAAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTTGTTGCCAGCGCGTGATGGCGGGAACTCAAAGGAGACTGCCGGTGACAAACCGGAGGAAGGCAGGGATGACGTCAAGTCATCATGGCCCTTACGGTCAGGGCTACACACGTGCTACAATGGGGCGTACAGAGGGAAGCGAGGCCGTGAGGCGGAGCGGAGCCCACAAAGCGTCCCACAGTTCGGATTGGAGTCTGCAACTCGACTCCATGAAGGCGGAATCGCTAGTAATCGCAAATCAGAATGTTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGATTGCACCAGAAGTGGCTTGCCTAACCCGTAAGGGAGGGCGGTTACCACGGTGTGGTTTATGACTGGGGTGAAGTCGTAACAAGGTAACCCTAGGGGAACCTGGGGTTGGATCACCTCCTT