| Firmicutes | Clostridia | Clostridiales | Lachnospiraceae |
Genome size: 3 241 294 bp
Genomic GC% content: 44.78 %
Scanning electron microscopy (10 000x magnification)
75
Au, 30 mA, 4 min
TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry
| Enzyme | PSM counts | Pathway |
| ATP-dependent 6-phosphofructokinase (EC=2.7.1.11) | 105 | Glycolysis / Gluconeogenesis |
| Triosephosphate isomerase (EC=5.3.1.1) | 82 | Glycolysis / Gluconeogenesis |
| Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) | 195 | Glycolysis / Gluconeogenesis |
| Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) | 278 | Glycolysis / Gluconeogenesis |
| Pyruvate, phosphate dikinase (EC=2.7.9.1) | 163 | Glycolysis / Gluconeogenesis |
| D-lactate dehydrogenase (EC=1.1.1.28) | 80 | Pyruvate metabolism |
| Pyruvate carboxylase (EC=6.4.1.1) | 193 | Citrate cycle (TCA cycle) |
| 2-oxoglutarate oxidoreductase (EC=1.2.7.3) | 131 | Citrate cycle (TCA cycle) |
| Acyl-CoA dehydrogenase, short-chain specific (EC=1.3.8.1) | 734 | Fatty acid degradation |
| NAD-specific glutamate dehydrogenase (EC=1.4.1.2) | 405 | Arginine biosynthesis |
| (R)-2-hydroxyisocaproyl-CoA dehydratase (EC=4.2.1.157) | 296 | |
| 2-aminoadipate transaminase (EC=2.6.1.39) | 160 | Lysine biosynthesis |
| Phosphoserine aminotransferase (EC=2.6.1.52) | 85 | Glycine, serine and threonine metabolism |
| NADH-dependent phenylglyoxylate dehydrogenase (EC=1.2.1.58) | 80 | Phenylalanine metabolism |
| Proton-translocating ferredoxin:NAD(+) oxidoreductase (EC=7.1.1.-) | 164 |
MS raw data: An75
culture medium: Wilkins-Chalgren Anaerobe Agar
16S rRNA sequence:
TAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATatTTagAATGAGAGCTTCGGCAGGATTTttaTCtATCTTAGTGGCGGACGGGTGAGTAACGTGTGGGCAACCTGCCCTGTACTGGGGAATAATCATTGGAAACGATGACTAATACCGCATGTGGTCCTCGGAAGGCATCTTcTGAGGAAGAAAGGATTTATTcGGTACAGGATGGGCCCGCATCTGATTAGCTAGTTGGTGAGATAACAGCCCACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGcGTGAAGGATGAAGGGTTTCGGCTCGTAAACTTCTATCAATAGGGAAGAAAgAAATGACGGTACCTAAATAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGCATGGTAAGCCAGATGTGAAAGCCTTGGGCTTAACCCGAGGATTGCATTTGGAACTATCAAGCTAGAGTACAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTTCTGGACTGAAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTCGGGGAGGAATCCTCGGTGCCGCAGCTAACGCAATAAGCACTCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGGCTTGACATCCCGATGACCGctCTAGAGATAGagCTTCTCTTCGGAGCATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCATCATTcAGTTGGGCACTCTGGAGAGACTGCCGTGGATAACACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTTGGGCTACACACGTGCTACAATGGCTGGTAACAAAGTGACGCaAgACGGCGACGTTAAGCAAATCACAAAAACCCAGTCCCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGAAGCACCCGAAGTCGGTGACCTAACCGTAAGGAAGGAGCCGCCGAAGGTGAAGCCAGTGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT