Lactobacillus gallinarum An115

Firmicutes Bacilli Lactobacillales Lactobacillaceae

Genome size: 2  039  377 bp

Genomic GC% content: 36.52 %

Scanning electron microscopy (3 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Asparagine synthetase (EC 6.3.5.4) 212 Alanine, aspartate and glutamate metabolism
Acetylornithine deacetylase (EC=3.5.1.16) 175 Arginine biosynthesis
Pyruvate oxidase (EC 1.2.3.3) 153 Glycolysis/Gluconeogenesis
Glucose-6-phosphate isomerase (EC 5.3.1.9) 211 Glycolysis/Gluconeogenesis
Fructose-bisphosphate aldolase (EC 4.1.2.13) 376 Glycolysis/Gluconeogenesis
Triosephosphate isomerase (EC 5.3.1.1) 204 Glycolysis/Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) 526 Glycolysis/Gluconeogenesis
Phosphoglycerate kinase (EC 2.7.2.3) 280 Glycolysis/Gluconeogenesis
Enolase (EC 4.2.1.11) 475 Glycolysis/Gluconeogenesis
Pyruvate kinase (EC 2.7.1.40) 557 Glycolysis/Gluconeogenesis
L-lactate dehydrogenase (EC 1.1.1.27) 483 Glycolysis/Gluconeogenesis
D-lactate dehydrogenase (EC 1.1.1.28) 248 Pyruvate metabolism
N-acetylglucosamine kinase  (EC 2.7.1.59) 155 Aminosugars metabolism
Aminopeptidase C (EC 3.4.22.40) 154 Protein degradation
6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) 124 Pentose cycle

MS raw data: An115

culture medium: Wilkins-Chalgren Anaerobe Agar + CN

16S rRNA sequence:

TGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCAGAACCAGCAGATTTACTTCGGTAATGACGCTGGGGACGCGAGCGGCGGATGGGTGAGTAACACGTGGGGAACCTGCCCCATAGTCTGGGATACCACTTGGAAACAGGTGCTAATACCGGATAAGAAAGCAGATCGCATGATCAGCTTATAAAAGGCGGCGTAAGCTGTCGCTATGGGATGGCCCCGCGGTGCATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTGGTGAAGAAGGATAGAGGTAGTAACTGGCCTTTATTTGACGGTAATCAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGAAAAATAAGTCTGATGTGAAAGCCCTCGGCTTAACCGAGGAACTGCATCGGAAACTGTTTTTCTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGCTAAGTGTTGGGAGGTTTCCGCCTCTCAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCTAGTGCCATCCTAAGAGATTAGGAGTTCCCTTCGGGGACGCTAAGACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTATTAGTTGCCAGCATTAAGTTGGGCACTCTAATGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGGCAGTACAACGAGAAGCGAGCCTGCGAAGGCAAGCGAATCTCTGAAAGCTGTTCTCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGCACGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGAAGTCTGCAATGCCCAAAGCCGGTGGCCTAACCTTCGGGAAGGAGCCGTCTAAGGCAGGGCAGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATCACCTCCTTT