Firmicutes | Clostridia | Clostridiales | Ruminococcaceae |
Genome size: 2 841 521 bp
Genomic GC% content: 62.40 %
Scanning electron microscopy (20 000x magnification)
TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry
Enzyme | PSM counts | Pathway |
Glutamate synthase (EC=1.4.1.13) | 136 | Alanine, aspartate and glutamate metabolism |
Acetyl-CoA acetyltransferase (EC=2.3.1.9) | 425 | Butyrate production |
3-hydroxybutyryl-CoA dehydrogenase (EC=1.1.1.157) | 913 | Butyrate production |
4-hydroxybutyryl-CoA dehydratase (EC=4.2.1.120) | 132 | Butyrate production |
Acyl-CoA dehydrogenase (EC=1.3.8.1) | 400 | Butyrate production |
Butyryl-CoA:acetate CoA-transferase (EC=2.8.3.8) | 156 | Butyrate production |
L-methionine gamma-lyase (EC=4.4.1.11) | 260 | Cysteine and methionine metabolism |
Pyrophosphate–fructose 6-phosphate 1-phosphotransferase (EC=2.7.1.90) | 171 | Glycolysis/Gluconeogenesis |
Fructose-bisphosphate aldolase (EC=4.1.2.13) | 135 | Glycolysis/Gluconeogenesis |
Triosephosphate isomerase (EC=5.3.1.1) | 154 | Glycolysis/Gluconeogenesis |
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) | 440 | Glycolysis/Gluconeogenesis |
Enolase (EC=4.2.1.11) | 136 | Glycolysis/Gluconeogenesis |
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) | 319 | Glycolysis/Gluconeogenesis |
Pyruvate, phosphate dikinase (EC=2.7.9.1) | 195 | Glycolysis/Gluconeogenesis |
3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (EC=3.7.1.22) | 170 | Inositol phosphate metabolism |
MS raw data: An411
culture medium: Yeast Casitone Fatty Acids Agar
16S rRNA sequence:
CTTAACACATGCAAGTCGAACGGAGCACCCTTGACTGAGGTTTCGGCCAAATGATAGGAATGCTTAGTGGCGGACTGGTGAGTAACGCGTGAGGAACCTGCCTTCCAGAGGGGGACAACAGTTGGAAACGACTGCTAATACCGCATGATATATTTGAGGGGCATCCCTTGGATATCAAAGATTTATCGCTGGAAGATGGCCTCGCGTCTGATTAGCTAGTAGGCGGGGTAACGGCCCACCTAGGCGACGATCAGTAGCCGGACTGAGAGGTTGACCGGCCACATTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGAAGGAAGAAGGCTTTCGGGTTGTAAACTTCTTTTGTCAGGGAACAGTAGAAGAGGGTACCTGACGAATAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGCTGGTAAGTCAGATGTGAAATCTGGAGGCTCAACCTCCAAACTGCATTTGAAACTGCTGGTCTTGAGTACCGGAGAGGTTATCGGAATTCCTTGTGTAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAGTGGCGAAGGCGGATAACTGGACGGCAACTGACGGTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGGATACTAGGTGTGCGGGGACTGACCCCCTGCGTGCCGCAGTTAACACAATAAGTATCCCACCTGGGGAGTACGATCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGGCTTGACATCCTGCTAACGAAGTAGAGATACATCAGGTGCCCTTCGGGGAAAGCAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTGTTAGTTGCTACGCAAGAGCACTCTAGCGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGTCCTGGGCTACACACGTAATACAATGGCGGTCAACAGAGGGAGGCAAAGCCGCGAGGCAGAGCAAACCCCCAAAAGCCGTCCCAGTTCGGATCGCAGGCTGCAACCCGCCTGCGTGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTCGGGAACACCCGAAGTCCGTAGCCTAACCGCAAGGGGGGCGCGGCCGAAGGTGGGTTCGATAATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT