Lactobacillus coleohominis An526

Firmicutes Bacilli Lactobacillales Lactobacillaceae

Genome size: 1 703 045 bp

Genomic GC% content: 39.10 %

Scanning electron microscopy (11 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Glutamate decarboxylase (EC 4.1.1.15) 452 Alanine, aspartate and glutamate metabolism
Glutamine synthetase (EC 6.3.1.2) 232 Arginine biosynthesis
2,3-butanediol dehydrogenase (EC 1.1.1.4) 397 Butyrate production
Sorbitol dehydrogenase (EC 1.1.1.14) 339 Fructose and mannose metabolism
Maltose phosphorylase (EC 2.4.1.8) 568 Starch and sucrose metabolism
Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) 494 Pentose phosphate pathway
6-phosphogluconate dehydrogenase (EC 1.1.1.44) 591 Pentose phosphate pathway
Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) 398 Pentose phosphate pathway
Phosphoglucomutase (EC5.4.2.6) 263 Glycolysis/Gluconeogenesis
Glucose-6-phosphate isomerase (EC 5.3.1.9) 254 Glycolysis/Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) 355 Glycolysis/Gluconeogenesis
Enolase (EC 4.2.1.11) 323 Glycolysis/Gluconeogenesis
Pyruvate kinase (EC 2.7.1.40) 271 Glycolysis/Gluconeogenesis
Alcohol dehydrogenase (EC 1.1.1.1) 579 Glycolysis/Gluconeogenesis
ATP synthase (EC 7.1.2.2) 367 Oxidative phosphorylation

MS raw data: An526

culture medium: Rogosa agar

16S rRNA sequence:

TGAGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATGCAAGTCGAACGCGTTGGCCCGACTGATTGATGATGCTTGCATCTGATTGACGACGGTTTACCAACGAGTGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCCAGAAGCGGGGGACAACATTTGGAAACAAGTGCTAATACCGCATAACAACGAAAACCACATGATTTTCGTTTAAAAGATGGTTTCGGCTATCACTTCTGGATGGGCCTGCGGCGCATTAGCTTGTTGGTGAGGTAACGGCTCACCAAGGCTGTGATGCGTAGCCGAGTTGAGAGACTGATCGGCCACAATGGAACTGAGACACGGTCCATACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGATGGAGCAACACCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAACTCTGTTGTTGAAGAAGAACGTGCGTGAGAGTAACTGTTCACGCAGTGACGGTATTCAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTCTAAGTCTGATGTGAAAGCCTTCGGCTTAACCGGAGAAGTGCATCGGAAACTGGATAACTTGAGTGCAAGAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTAGCTTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGCTAGGTGTTGGAGGGTTTCCGCCCTTCAGTGCCGGAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATCTTGCGCTAACCTCAGAGATGAGGCGTTCCCTTCGGGGACGCAATGACAGGTGGTGCATGGTCGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTACTAGTTGCCAGCATTCAGTTGGGCACTCTAGTGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAGATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAACGAGCAGCGAACTCGCGAGGGCAAGCTAATCTCTTAAAACCGTTCTCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTTGCAATGCCCAAAGCCGGTGGCCTAACCTTCGGGAAGGAGCCGTCTAAGGCAGGGCAGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATCACCTCCTTT