Firmicutes | Clostridia | Clostridiales | Ruminococcaceae |
Genome size: 3 018 026 bp
Genomic GC% content: 56.20 %
Scanning electron microscopy (20 000x magnification)
TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry
Enzyme | PSM counts | Pathway |
6-phospho-beta-glucosidase (EC 3.2.1.86) | 201 | |
6-phosphofructokinase (EC 2.7.1.11) | 219 | Glycolysis |
Fructose-bisphosphate aldolase class II (EC 4.1.2.13) | 284 | Glycolysis |
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) | 1514 | Glycolysis |
Phosphoglycerate kinase (EC 2.7.2.3) | 304 | Glycolysis |
Enolase (EC 4.2.1.11) | 433 | Glycolysis |
Pyruvate kinase (EC 2.7.1.40) | 319 | Glycolysis |
Pyruvate,phosphate dikinase (EC 2.7.9.1) | 273 | |
Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-) | 816 | |
Electron transfer flavoprotein, alpha subunit | 327 | |
Acetyl-CoA acetyltransferase (EC 2.3.1.9) | 337 | Butyrate production |
Butyryl-CoA dehydrogenase (EC 1.3.99.2) | 292 | Butyrate production |
Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) | 237 | Pyridine biosynthesis |
Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21) | 186 | Glycogen synthesis |
Periplasmic [Fe] hydrogenase (EC 1.12.7.2) | 185 |
MS raw data: An85
culture medium: Wilkins-Chalgren Anaerobe Agar + CN
16S rRNA sequence: TATTGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAACGGATTGAGGATTCGTCCAAATGAAGTTGAGGAAAGTGGCGGACGGGTGAGTAACGCGTGAGGAACCTGCCTCAGAGTGGGGAATAACAGTTAGAAATGACTGCTAATACCGCATAATGTAGCGAGATCGCATGAtCTTGCTACCAAAGATTTATCGCTCTGAGATGGCCTCGCGTCTGATTAGCTAGTTGGCGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGACTGAGAGGTTGGCCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGAAGGAAGAAGGCTTTCGGGTTGTAAACTTCTTTTGTCAGGGACGAAGCAAGTGACGGTACCTGACGAATAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACCACGGGCTCAACCTGTGGCCTGCATTTGAAACTGTAGTTCTTGAGTGTCGGAGAGGCAATCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGATTGCTGGACGATAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGGGGACTGACCCCCTCCGTGCCGCAGTTAACACAATAAGTATCCCACCTGGGGAGTACGATCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGGCTTGACATCCTACTAACGAAGCAGAGATGCATTAGGTGCCCTTCGGGGAAAGTAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTGTTAGTTGCTACGCAAGAGCACTCTAGCGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGTCCTGGGCCACACACGTACTACAATGGCGGTTAACAAAGAGAGGCAATACCGCGAGGTGGAGCAAATCTCAAAAAGCCGTCCCAGTTCGGATTGCAGGCTGCAACCCGCCTGCATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTCGGGAACACCCGAAGTCCGTAGCCTAACCGCAAGGGGGGCGCGGCCGAAGGTGGGTTCGATAATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT