Pseudoflavonifractor capillosus An85

Firmicutes Clostridia Clostridiales Ruminococcaceae

Velikost genomu: 3 018 026 bp

Obsah GC% v genomu: 56,20 %

Skenovací elektronová mikroskopie (zvětšení 20 000x)

 

TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií

Enzyme PSM counts Pathway
6-phospho-beta-glucosidase (EC 3.2.1.86) 201
6-phosphofructokinase (EC 2.7.1.11) 219 Glycolysis
Fructose-bisphosphate aldolase class II (EC 4.1.2.13) 284 Glycolysis
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) 1514 Glycolysis
Phosphoglycerate kinase (EC 2.7.2.3) 304 Glycolysis
Enolase (EC 4.2.1.11) 433 Glycolysis
Pyruvate kinase (EC 2.7.1.40) 319 Glycolysis
Pyruvate,phosphate dikinase (EC 2.7.9.1) 273
Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-) 816
Electron transfer flavoprotein, alpha subunit 327
Acetyl-CoA acetyltransferase (EC 2.3.1.9) 337 Butyrate production
Butyryl-CoA dehydrogenase (EC 1.3.99.2) 292 Butyrate production
Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) 237 Pyridine biosynthesis
Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21) 186 Glycogen synthesis
Periplasmic [Fe] hydrogenase (EC 1.12.7.2) 185

MS raw data: An85

kultivační médium: Wilkins-Chalgren Anaerobe Agar + CN

Sekvence 16S rRNA: TATTGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAACGGATTGAGGATTCGTCCAAATGAAGTTGAGGAAAGTGGCGGACGGGTGAGTAACGCGTGAGGAACCTGCCTCAGAGTGGGGAATAACAGTTAGAAATGACTGCTAATACCGCATAATGTAGCGAGATCGCATGAtCTTGCTACCAAAGATTTATCGCTCTGAGATGGCCTCGCGTCTGATTAGCTAGTTGGCGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGACTGAGAGGTTGGCCGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGACCCAGCAACGCCGCGTGAAGGAAGAAGGCTTTCGGGTTGTAAACTTCTTTTGTCAGGGACGAAGCAAGTGACGGTACCTGACGAATAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGATGTGAAAACCACGGGCTCAACCTGTGGCCTGCATTTGAAACTGTAGTTCTTGAGTGTCGGAGAGGCAATCGGAATTCCGTGTGTAGCGGTGAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGATTGCTGGACGATAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGGGGACTGACCCCCTCCGTGCCGCAGTTAACACAATAAGTATCCCACCTGGGGAGTACGATCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGGCTTGACATCCTACTAACGAAGCAGAGATGCATTAGGTGCCCTTCGGGGAAAGTAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATTGTTAGTTGCTACGCAAGAGCACTCTAGCGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGTCCTGGGCCACACACGTACTACAATGGCGGTTAACAAAGAGAGGCAATACCGCGAGGTGGAGCAAATCTCAAAAAGCCGTCCCAGTTCGGATTGCAGGCTGCAACCCGCCTGCATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTCGGGAACACCCGAAGTCCGTAGCCTAACCGCAAGGGGGGCGCGGCCGAAGGTGGGTTCGATAATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT