Anaerotignum (Clostridium) lactatifermentans An75

Firmicutes Clostridia Clostridiales Lachnospiraceae

Genome size: 3  241  294 bp

Genomic GC% content: 44.78 %

Scanning electron microscopy (10 000x magnification)

75
Au, 30 mA, 4 min

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
ATP-dependent 6-phosphofructokinase (EC=2.7.1.11) 105 Glycolysis / Gluconeogenesis
Triosephosphate isomerase (EC=5.3.1.1) 82 Glycolysis / Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 195 Glycolysis / Gluconeogenesis
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) 278 Glycolysis / Gluconeogenesis
Pyruvate, phosphate dikinase (EC=2.7.9.1) 163 Glycolysis / Gluconeogenesis
D-lactate dehydrogenase (EC=1.1.1.28) 80 Pyruvate metabolism
Pyruvate carboxylase (EC=6.4.1.1) 193 Citrate cycle (TCA cycle)
2-oxoglutarate oxidoreductase (EC=1.2.7.3) 131 Citrate cycle (TCA cycle)
Acyl-CoA dehydrogenase, short-chain specific (EC=1.3.8.1) 734 Fatty acid degradation
NAD-specific glutamate dehydrogenase (EC=1.4.1.2) 405 Arginine biosynthesis
(R)-2-hydroxyisocaproyl-CoA dehydratase (EC=4.2.1.157) 296
2-aminoadipate transaminase (EC=2.6.1.39) 160 Lysine biosynthesis
Phosphoserine aminotransferase (EC=2.6.1.52) 85 Glycine, serine and threonine metabolism
NADH-dependent phenylglyoxylate dehydrogenase (EC=1.2.1.58) 80 Phenylalanine metabolism
Proton-translocating ferredoxin:NAD(+) oxidoreductase (EC=7.1.1.-) 164

MS raw data: An75

culture medium: Wilkins-Chalgren Anaerobe Agar

16S rRNA sequence:

TAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATatTTagAATGAGAGCTTCGGCAGGATTTttaTCtATCTTAGTGGCGGACGGGTGAGTAACGTGTGGGCAACCTGCCCTGTACTGGGGAATAATCATTGGAAACGATGACTAATACCGCATGTGGTCCTCGGAAGGCATCTTcTGAGGAAGAAAGGATTTATTcGGTACAGGATGGGCCCGCATCTGATTAGCTAGTTGGTGAGATAACAGCCCACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGcGTGAAGGATGAAGGGTTTCGGCTCGTAAACTTCTATCAATAGGGAAGAAAgAAATGACGGTACCTAAATAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGCATGGTAAGCCAGATGTGAAAGCCTTGGGCTTAACCCGAGGATTGCATTTGGAACTATCAAGCTAGAGTACAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTTCTGGACTGAAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTCGGGGAGGAATCCTCGGTGCCGCAGCTAACGCAATAAGCACTCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGGCTTGACATCCCGATGACCGctCTAGAGATAGagCTTCTCTTCGGAGCATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCATCATTcAGTTGGGCACTCTGGAGAGACTGCCGTGGATAACACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTTGGGCTACACACGTGCTACAATGGCTGGTAACAAAGTGACGCaAgACGGCGACGTTAAGCAAATCACAAAAACCCAGTCCCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGAAGCACCCGAAGTCGGTGACCTAACCGTAAGGAAGGAGCCGCCGAAGGTGAAGCCAGTGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT