Anaerotruncus colihominis An174

Firmicutes Clostridia Clostridiales Ruminococcaceae

Genome size: 4  104  028 bp

Genomic GC% content: 53.46 %

Scanning electron microscopy (20 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Fructose-bisphosphate aldolase (EC=4.1.2.13) 150 Glycolysis/Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 355 Glycolysis/Gluconeogenesis
Phosphoglycerate kinase (EC=2.7.2.3) 194 Glycolysis/Gluconeogenesis
Pyruvate, phosphate dikinase (EC=2.7.9.1) 241 Glycolysis/Gluconeogenesis
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) 324 Glycolysis/Gluconeogenesis
Aldehyde-alcohol dehydrogenase (EC=1.1.1.1) 174 Glycolysis/Gluconeogenesis
Oxaloacetate decarboxylase (EC=7.2.4.2) 184 Pyruvate metabolism
3-oxoacyl-[acyl-carrier-protein] reductase (EC=1.1.1.100) 194 Butyrate production
Acyl-CoA dehydrogenase (EC=1.3.8.1) 230 Butyrate production
3-hydroxybutyryl-CoA dehydrogenase (EC=1.1.1.157) 157 Butyrate production
Acetyl-CoA acetyltransferase (EC=2.3.1.9) 228 Butyrate production
Glycerol dehydrogenase (EC=1.1.1.6) 267 Glycerolipid metabolism
4-hydroxy-tetrahydrodipicolinate synthase (EC=4.3.3.7) 256 Lysine biosynthesis
4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC=1.17.7.4) 170 Terpenoid backbone biosynthesis
Tryptophanase (EC=4.1.99.1) 174 Tryptophan metabolism

 

16S rRNA sequence:

AAAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTACGTTTTGAAGTTTTCGGATGGATGAATGTAAGCTTAGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGCCTTTCAGAGGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATGATGTTGCGGGGGCACATGCCCCTGCAACCAAAGGAGCAATCCGCTGAAAGATGGGCTCGCGTCCGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGAAGACGGTCTTCGGATTGTAAACCTCTGTCTTTGGGGAAGAAAATGACGGTACCCAAAGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTAGAATGTTAAATCCATCGGCTCAACCGGTGGCTGCGTTCTAAACTGCCGTTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGGATGCATAGCCTAGAGATAGGTGAAGCCCTTCGGGGCATCCAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATTAGTTGCTACGCAAGAGCACTCTAATGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCACTAAAACAGAGGGCGGCGACACCGCGAGGTGAAGCGAATCCCGAAAAAGTGTCTCAGTTCAGATTGCAGGCTGCAACCCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCGGTAACACCCGAAGCCAGTAGCCTAACCGCAAGGGGGGCGCTGTCGAAGGTGGGATTGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT