Bacillus licheniformis An441

Firmicutes Bacilli Bacillales Bacillaceae

Genome size: 4 243 165 bp

Genomic GC% content: 45.90 %

Scanning electron microscopy (20 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Carbamate kinase (EC 2.7.2.2) 656 Arginine biosynthesis
Ornithine carbamoyltransferase (EC 2.1.1.3) 505 Arginine biosynthesis
Arginine deiminase (EC 3.5.3.6) 943 Arginine biosynthesis
Isocitrate dehydrogenase (EC 1.1.1.42) 340 Citrate cycle (TCA cycle)
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) 899 Glycolysis/Gluconeogenesis
Phosphoglycerate kinase (EC 2.7.2.3) 407 Glycolysis/Gluconeogenesis
Phosphoglycerate mutase (EC 5.4.2.11) 269 Glycolysis/Gluconeogenesis
Enolase (EC 4.2.1.11) 366 Glycolysis/Gluconeogenesis
Pyruvate kinase (EC 2.7.1.40) 612 Glycolysis/Gluconeogenesis
Alcohol dehydrogenase (EC 1.1.1.1) 797 Glycolysis/Gluconeogenesis
Respiratory nitrate reductase (EC 1.7.1.1) 431 Nitrogen metabolism
ATP synthase (EC 7.1.2.2) 626 Oxidative phosphorylation
Pyruvate formate-lyase (EC 2.3 .1 .54) 892 Pyruvate metabolism
Maltose-6ʼ-phosphate glucosidase (EC 3.2.1.22) 665 Starch and sucrose metabolism
Beta-glucosidase (EC 3.2.1.21) 450 Starch and sucrose metabolism

MS raw data: An441

culture medium: Wilkins-Chalgren Anaerobe Agar

16S rRNA sequence:

AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACCGACGGGAGCTTGCTCCCTTAGGTCAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGATTGAACCGCATGGTTCAATCATAAAAGGTGGCTTTTAGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGAGGCTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT