Bacteroides helcogenes ET71

Bacteroidetes Bacteroidia Bacteroidales Bacteroidaceae

Genome size: 3 628 556 bp

Genomic GC% content: 50.60 %

Scanning electron microscopy (20 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Fumarate reductase (EC=1.3.5.4) 136 Anaerobic respiration
Malate dehydrogenase (EC=1.1.1.37) 259 Citrate cycle (TCA cycle)
Fumarate hydratase class I, anaerobic (EC=4.2.1.2) 130 Citrate cycle (TCA cycle)
Beta-galactosidase (EC=3.2.1.23) 167 Galactose metabolism
Phosphoglucomutase (EC=5.4.2.2) 161 Glycolysis/Gluconeogenesis
Pyrophosphate–fructose 6-phosphate 1-phosphotransferase (EC=2.7.1.90) 365 Glycolysis/Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 2100 Glycolysis/Gluconeogenesis
Phosphoenolpyruvate carboxykinase (ATP) (EC=4.1.1.49) 343 Glycolysis/Gluconeogenesis
Pyruvate kinase (EC=2.7.1.40) 126 Glycolysis/Gluconeogenesis
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) 332 Glycolysis/Gluconeogenesis
Pyruvate, phosphate dikinase (EC=2.7.9.1) 101 Glycolysis/Gluconeogenesis
NADP-dependent malic enzyme (EC=1.1.1.40) 129 Pyruvate metabolism
Glycogen phosphorylase (EC=2.4.1.1) 181 Starch and sucrose metabolism
Methylmalonyl-CoA mutase (EC=5.4.99.2) 171 Valine, leucine and isoleucine degradation
Propionyl-CoA carboxylase (EC=6.4.1.3) 103 Valine, leucine and isoleucine degradation

MS raw data: ET71

culture medium: chocolate agar

16S rRNA sequence:

ATTACAGTGAAGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCATGAGCTTAGCTTGCTAAGTTTGATGGCGACCGGCGCACGGGTGAGTAACGCGTATCCGACCTGCCTTCCACTCGGGGATAGCCTTTCGAAAGAAAGATTAATACCCGATGGCATAAGCGAACTGCCTGGTTTGCTTATTAAAGATTCATCGGTGGAAGATGGGGATGCGTTCCATTAGCTTGTTGGCGGGGTAACGGCCCACCAAGGCTACGATGGATAGGGGTTCTGAGAGGAAGGTCCCCCACATTGGAACTGAGACACGGTCCAAACTCCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGCGAGGCTGAACCAGCCAAGTAGCGTGAAGGATGACTGCCCTATGGGTTGTAAACTTCTTTTATAAGGGAATAAAGTGTTGTACGTGTACGATATTGCATGTACCTTATGAATAAGCATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGCGATTAAGTCAGTTGTGAAAGTTCGCGGCTCAACCGTGAAATTGCAGTTGAAACTGGTTGCCTTGAGTGCGGCAGAGGCAGGCGGAATTCGTGGTGTAGCGGTGAAATGCTTAGATATCACGAAGAACTCCGATTGCGAAGGCAGCTTGCTGGACCGTAACTGACGTTGAGGCTCGAAAGTGTGGGTATCAAACAGGATTAGATACCCTGGTAGTCCACACAGTAAACGATGGACACTCGCTGTTGGCGATATACAGTCAGCGGCTTAGCGAAAGCGTTAAGTGTCCCACCTGGGGAGTACGCCGGCAACGGTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGAGGAACATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCCGGGCTTGAACTGTGCCTGACAGATTCGGAAACGGATATTCCCTTCGGGGCAGGCATGGAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTCGGCTTAAGTGCCATAACGAGCGCAACCCTTACTGCCAGTTGCCATCGGGTAATGCCGGGCACTCTGGTAGGACTGCCGTCGTAAGATGTGAGGAAGGTGGGGATGACGTCAAATCAGCACGGCCCTTACGTCCGGGGCTACACACGTGTTACAATGGGGGGTACAGCAGGCCGCTACCCGGCGACGGGATGCCAATCCTGAAAGCCCCTCTCAGTTCGGACTGGAGTCTGCAACCCGACTCCACGAAGCTGGATTCGCTAGTAATCGCGCATCAGCCACGGCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGCCATGAAAGCCGGGGGTACCTGAAGTGCGTAACCGCGAGGGGCGCCCTAGGGTAAAACCGGTGATTGGGGCTAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAACACCTCCTTTCTG