Butyricicoccus pullicaecorum An179

Firmicutes Clostridia Clostridiales Ruminococcaceae

Genome size: 3 474 625 bp

Genomic GC% content: 53.61 %

Scanning electron microscopy (11 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
6-phospho-beta-glucosidase (EC=3.2.1.86) 214 Glycolysis
Pyrophosphate–fructose 6-phosphate 1-phosphotransferase (EC=2.7.1.90) 267 Glycolysis
Fructose-bisphosphate aldolase (EC=4.1.2.13) 234 Glycolysis
Triosephosphate isomerase (EC=5.3.1.1) 473 Glycolysis
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 902 Glycolysis
2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC=5.4.2.12) 180 Glycolysis
Enolase (EC=4.2.1.11) 261 Glycolysis
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) 579 Glycolysis
Pyruvate kinase (EC=2.7.1.40) 314 Glycolysis
Acetyl-CoA acetyltransferase (EC=2.3.1.9) 392 Butyrate production
Acyl-CoA dehydrogenase (EC=1.3.8.1) 464 Butyrate production
Short-chain-enoyl-CoA hydratase (EC=4.2.1.150) 312 Butyrate production
3-hydroxybutyryl-CoA dehydrogenase (EC=1.1.1.157) 342 Butyrate production
4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA-Delta-isomerase (EC=4.2.1.120) 163 Butyrate production
NADP-specific glutamate dehydrogenase (EC=1.4.1.4) 182 Arginine biosynthesis

MS raw data: An179

culture medium: Wilkins-Chalgren Anaerobe Agar + CN

16S rRNA sequence:

TTTAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGTTTGAGGAAATCCTTCGGGATGGAATCTTCCAACTTAGTGGCGGACGGGTGAGTAACGCGTGAGCAATCTGCCTTTCAGAGGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATAATGCATCGAATTCGCATGTTTTTGATGCCAAAGATTTTATCGCTGAAAGATGAGCTCGCGTCTGATTAGCTAGTTGGCGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGATTGATGAAGGTCTTCGGATTGTAAAAATCTTTAATCAGGGACGAAACAAATGACGGTACCTGAAGAATAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGCTTGTAAGTTGGAAGTGAAATCTCGGGGCTTAACCCCGAAACTGCTTTCAAAACTGCGAGTCTTGAGTGATGGAGAGGCAGGCGGAATTCCCAGTGTAGCGGTGAAATGCGTAGATATTGGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACATTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGAGGTATTGACCCCTTCCGTGCCGGAGTTAACACAATAAGTATCCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCGATGACCGCTCTAGAGATAGGGCTTCTCTTCGGAGCATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTACGGTTAGTTGCTACGCAAGAGCACTCTAGCCGGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGACTTGGGCTACACACGTACTACAATGGCAGTCATACAGAGGGAAGCAAAACCGCGAGGTGGAGCAAATCCCTAAAAGCTGTCCCAGTTCAGATTGCAGGCTGCAACTCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGCCGGTAATACCCGAAGTCCGTAGTCTAACCGCAAGGAGGACGCGGCCGAAGGTAGGACTGGTAATTAGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT