| Firmicutes | Clostridia | Clostridiales | Lachnospiraceae |
Genome size: 3 190 622 bp
Genomic GC% content: 46.76 %
Scanning electron microscopy (20 000x magnification)
TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry
| Enzyme | PSM counts | Pathway |
| L-aspartate oxidase (EC 1.4.3.16) | 345 | Alanine, aspartate and glutamate metabolism |
| Glutamate synthase [NADPH] (EC 1.4.1.13) | 480 | Alanine, aspartate and glutamate metabolism |
| Glutamate dehydrogenase (EC 1.4.1.4) | 280 | Alanine, aspartate and glutamate metabolism |
| Glutamine synthetase type III (EC 6.3.1.2) | 461 | Alanine, aspartate and glutamate metabolism |
| Fructose-bisphosphate aldolase class II (EC 4.1.2.13) | 291 | Glycolysis/Gluconeogenesis |
| Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) | 1081 | Glycolysis/Gluconeogenesis |
| Phosphoglycerate kinase (EC 2.7.2.3) | 291 | Glycolysis/Gluconeogenesis |
| Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) | 733 | Glycolysis/Gluconeogenesis |
| Pyruvate,phosphate dikinase (EC 2.7.9.1) | 766 | Glycolysis/Gluconeogenesis |
| Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-) | 752 | Glycolysis/Gluconeogenesis |
| Alcohol dehydrogenase (EC 1.1.1.1) | 245 | Glycolysis/Gluconeogenesis |
| CoB-CoM heterodisulfide reductase (EC 1.8.98.1) | 259 | Methane metabolism |
| Pyruvate formate-lyase (EC 2.3.1.54) | 273 | Pyruvate metabolism |
| Malic enzyme (EC 1.1.1.40) | 262 | Pyruvate metabolism |
| Ketol-acid reductoisomerase (EC 1.1.1.86) | 248 | Valine, leucine and isoleucine biosynthesis |
MS raw data: An298
culture medium: Wilkins-Chalgren Anaerobe Agar
16S rRNA sequence:
CGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTCTGCTTTGATTCTTCGGATGAAGAGCATTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGCACCGCATGGTGCAGGGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGCGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAACTGACGGTACCTGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTTCATCTAGAGTGCTGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGCCCTTGACATCCGAATGACCGGTGAGTAATGTCACCTTTCCTTCGGGACATTCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCAGTCCGGCTGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGGCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCAGAGCCGCGAGGCCGAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT