Clostridium glycyrrhizinilyticum An298

Firmicutes Clostridia Clostridiales Lachnospiraceae

Genome size: 3 190 622 bp

Genomic GC% content: 46.76 %

Scanning electron microscopy (20 000x magnification)

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
L-aspartate oxidase (EC 1.4.3.16) 345 Alanine, aspartate and glutamate metabolism
Glutamate synthase [NADPH] (EC 1.4.1.13) 480 Alanine, aspartate and glutamate metabolism
Glutamate dehydrogenase (EC 1.4.1.4) 280 Alanine, aspartate and glutamate metabolism
Glutamine synthetase type III (EC 6.3.1.2) 461 Alanine, aspartate and glutamate metabolism
Fructose-bisphosphate aldolase class II (EC 4.1.2.13) 291 Glycolysis/Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) 1081 Glycolysis/Gluconeogenesis
Phosphoglycerate kinase (EC 2.7.2.3) 291 Glycolysis/Gluconeogenesis
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) 733 Glycolysis/Gluconeogenesis
Pyruvate,phosphate dikinase (EC 2.7.9.1) 766 Glycolysis/Gluconeogenesis
Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-) 752 Glycolysis/Gluconeogenesis
Alcohol dehydrogenase (EC 1.1.1.1) 245 Glycolysis/Gluconeogenesis
CoB-CoM heterodisulfide reductase (EC 1.8.98.1) 259 Methane metabolism
Pyruvate formate-lyase (EC 2.3.1.54) 273 Pyruvate metabolism
Malic enzyme (EC 1.1.1.40) 262 Pyruvate metabolism
Ketol-acid reductoisomerase (EC 1.1.1.86) 248 Valine, leucine and isoleucine biosynthesis

MS raw data: An298

16S rRNA sequence:

CGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTCTGCTTTGATTCTTCGGATGAAGAGCATTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGCACCGCATGGTGCAGGGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGCGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAACTGACGGTACCTGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTTCATCTAGAGTGCTGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGCCCTTGACATCCGAATGACCGGTGAGTAATGTCACCTTTCCTTCGGGACATTCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCAGTCCGGCTGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGGCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCAGAGCCGCGAGGCCGAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT