Lactobacillus ingluviei An777

Firmicutes Bacilli Lactobacillales Lactobacillaceae

Genome size: 2 021 907 bp

Genomic GC% content: 50.30 %

Scanning electron microscopy (20 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Xylulose-5-phosphate phosphoketolase (EC=4.1.2.9) 401 Pentose phosphate pathway
6-phosphogluconate dehydrogenase, decarboxylating (EC=1.1.1.44) 347 Pentose phosphate pathway
Aldehyde-alcohol dehydrogenase (EC=1.1.1.1) 292
Enolase 2 (EC=4.2.1.11) 207 Glycolysis / Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 205 Glycolysis / Gluconeogenesis
Pyruvate kinase (EC=2.7.1.40) 189 Glycolysis / Gluconeogenesis
L-lactate dehydrogenase (EC=1.1.1.27) 86 Glycolysis / Gluconeogenesis
Diacetyl reductase [(S)-acetoin forming] (EC=1.1.1.304) 134 Butanoate metabolism
Glucose-6-phosphate 1-dehydrogenase (EC=1.1.1.363) 128 Pentose phosphate pathway
Asparagine synthetase [glutamine-hydrolyzing] 1 (EC=6.3.5.4) 117 Alanine, aspartate and glutamate metabolism
Glutamine synthetase (EC=6.3.1.2) 105 Alanine, aspartate and glutamate metabolism
Phosphoglucomutase (EC=5.4.2.2) 104 Glycolysis / Gluconeogenesis
Ornithine carbamoyltransferase (EC=2.1.3.3) 97  Arginine biosynthesis
NADP-specific glutamate dehydrogenase (EC=1.4.1.4) 92 Arginine biosynthesis
Acetate kinase (EC=2.7.2.1) 83

MS raw data: An777

16S rRNA sequence:

TATGAGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATGCAAGTCGAACGCGTTGGCCCAACTGATTGATTGTGCTTGCACAGGATTGACGTTGGTTTCCCAACGAGTGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCCCGAAGCGGGGGACAACATTTGGAAACAAGTGCTAATACCGCATAACAACTTTGACCGCCTGGTCAAAGCTTAAAAGATGGTTTCGGCTATCACTTCGGGATGGGCCTGCGGCGCATTAGCTTGTTGGTGGGGTAACGGCCTACCAAGGCGATGATGCGTAGCCGAGTTGAGAGACTGATCGGCCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGATGGAGCAACACCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAGCTCTGTTGTTGAAGAAGAATGTGCAAGAGAGTAACTGTTCTTGTATTGACGGTATTCAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTCTAAGTCTGATGTGAAAGCCTTCGGCTTAACCGAAGAAGTGCATCGGAAACTGGATGACTTGAGTGCAGAAGAGGGCAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGCCTGGTCTGTAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGCTAGGTGTTGGAGGGTTTCCGCCCTTCAGTGCCGGAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATCTTGCGCCAACCCTAGAGATAGGGCGTTCCCTTCGGGGACGCAATGACAGGTGGTGCATGGTCGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTACTAGTTGCCAGCATTCAGTTGGGCACTCTAGTGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAGATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAACGAGTCGCGAACTCGCGAGGGCAAGCTAATCTCTTAAAACCGTTCTCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTTGCAATGCCCAAAGTCGGTGGGGTAACCTTTTGGAGCCAGCCGCCTAAGGCAGGGCAGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATCACCTCCTTT