Lactobacillus (johnsonii) gasseri An197

Firmicutes Bacilli Lactobacillales Lactobacillaceae

Genome size: 1 786 561 bp

Genomic GC% content: 34.60 %

Scanning electron microscopy (4 500x magnification)

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
L-asparaginase (EC 3.5.1.1) 73 Alanine, aspartate and glutamate metabolism
Glutamine synthetase type I (EC 6.3.1.2) 59 Arginine biosynthesis
N-acetylglucosamine kinase (EC 2.7.1.59) 96 Amino sugar and nucleotide sugar metabolism
Phosphoglucosamine mutase (EC 5.4.2.10) 79 Amino sugar and nucleotide sugar metabolism
Glucose-6-phosphate isomerase (EC 5.3.1.9) 66 Glycolysis/Gluconeogenesis
Fructose-bisphosphate aldolase class II (EC 4.1.2.13) 120 Glycolysis/Gluconeogenesis
Triosephosphate isomerase (EC 5.3.1.1) 187 Glycolysis/Gluconeogenesis
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase  (EC 1.2.1.12) 255 Glycolysis/Gluconeogenesis
Phosphoglycerate kinase (EC 2.7.2.3) 188 Glycolysis/Gluconeogenesis
Phosphoglycerate mutase (EC 5.4.2.11) 194 Glycolysis/Gluconeogenesis
Enolase (EC 4.2.1.11) 194 Glycolysis/Gluconeogenesis
Pyruvate kinase (EC 2.7.1.40) 259 Glycolysis/Gluconeogenesis
L-lactate dehydrogenase (EC 1.1.1.27) 127 Glycolysis/Gluconeogenesis
ATP synthase beta chain (EC 3.6.3.14) 180 Oxidative phosphorylation
Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) 58 Pentose phosphate pathway

 

16S rRNA sequence:

TGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCTTGCCTAGATGATTTTAGTGCTTGCACTAAATGAAACTAGATACAAGCGAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCCAAGAGACTGGGATAACACCTGGAAACAGATGCTAATACCGGATAACAACACTAGACGCATGTCTAGAGTTTGAAAGATGGTTCTGCTATCACTCTTGGATGGACCTGCGGTGCATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCAATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAGCTCTGTTGGTAGTGAAGAAAGATAGAGGTAGTAACTGGCCTTTATTTGACGGTAATTACTTAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCAGGCGGTTCAATAAGTCTGATGTGAAAGCCTTCGGCTCAACCGGAGAATTGCATCAGAAACTGTTGAACTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTCTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGCTAAGTGTTGGGAGGTTTCCGCCTCTCAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCAGTGCAAACCTAAGAGATTAGGTGTTCCCTTCGGGGACGCTGAGACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCATTAGTTGCCATCATTAAGTTGGGCACTCTAATGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAACGAGAAGCGAACCTGCGAAGGCAAGCGGATCTCTTAAAGCCGTTCTCAGTTCGGACTGTAGGCTGCAACTCGCCTACACGAAGCTGGAATCGCTAGTAATCGCGGATCAGCACGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTCTGTAACACCCAAAGCCGGTGGGATAACCTTTATAGGAGTCAGCCGTCTAAGGTAGGACAGATGATTAGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATCACCTCCTTT