Ligilactobacillus (Lactobacillus) salivarius An63

Firmicutes Bacilli Lactobacillales Lactobacillaceae

Genome size: 1 826 390 bp

Genomic GC% content: 32.76 %

Scanning electron microscopy (20 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
D-arabitol-phosphate dehydrogenase (EC=1.1.1.301) 74 Saccharide degradation
Beta-galactosidase (EC=3.2.1.23) 110 Saccharide degradation
Alpha-galactosidase (EC=3.2.1.22) 103 Saccharide degradation
Phosphoglucomutase (EC=5.4.2.2) 95  Glycolysis / Gluconeogenesis
Fructose-bisphosphate aldolase (EC=4.1.2.13) 83  Glycolysis / Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 140  Glycolysis / Gluconeogenesis
Enolase (EC=4.2.1.11) 140  Glycolysis / Gluconeogenesis
Formate acetyltransferase (EC=2.3.1.54) 237  Glycolysis / Gluconeogenesis
Pyruvate kinase (EC=2.7.1.40) 215  Glycolysis / Gluconeogenesis
Aldehyde-alcohol dehydrogenase (EC=1.1.1.1) 214  Glycolysis / Gluconeogenesis
Xylulose-5-phosphate phosphoketolase (EC=4.1.2.9) 165 Pentose phosphate pathway
Glucose-6-phosphate 1-dehydrogenase (EC=1.1.1.49) 71 Pentose phosphate pathway
Pyruvate carboxylase (EC=6.4.1.1) 87 Citrate cycle (TCA cycle)
Glutamine synthetase (EC=6.3.1.2) 83 Arginine biosynthesis
Asparagine synthetase (EC=6.3.5.4) 76 Alanine, aspartate and glutamate metabolism

MS raw data: An63

culture medium: Wilkins-Chalgren Anaerobe Agar

16S rRNA sequence:

AATGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAAACTTTCTTACACCGAATGCTTGCATTCAcCGTAAGAAGTTGAGTGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTAAAAGAAGGGGATAACACTTGGAAACAGGTGCTAATACCGTATaTCTCTaAGGaTCGCATGAtccTTAGAtGAAAGATGGTTCTGCTATCGcTTTTAGATGGACCCGCGGCGTATTAACTAGTTGGTGGGGTAACGGCCTACCAAGGTGATGATACGTAGCCGAACTGAGAGGTTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGATGGAGCAACGCCGCGTGAGTGAAGAAGGTCTTCGGATCGTAAAACTCTGTTGTTAGAGAAGAACACGAGTGAGAGTAACTGTTCATTCGATGACGGTATCTAACCAGCAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAACGCAGGCGGTCTTTTAAGTCTGATGTGAAAGCCTTCGGCTTAACcGGAGTAGTGCATTGGAAACTGGAAGACTTGAGTGCAGAAGAGGAGAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGTGGCGAAAGCGGCTCTCTGGTCTGTAACTGACGCTGAGGTTCGAAAGCGTGGGTAGCAAACAgGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGCTAGGTGTTGGAGGGTTTCCgCCCTTCAGTGCCGCAGCTAACGCAATAAGCATTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTTTGACCACCTAAGAGATTAGGtTTTCCtTTCGGGGACAAAGTGACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTGTCAGTTGCCAGCATTAAGTTGGGCACTCTGGCGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAACGAGTCGCaAGACCGCGAGGTTTAGCTAATCTCTTAAAGCCGTTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTACATGAAGTCGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGTTTGTAACACCCAAAGCCGGTGGGGTAACCGCAAGGAGCCAGCCGTCTAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATCACCTCCTTT