Limosilactobacillus (Lactobacillus) reuteri An71

Firmicutes Bacilli Lactobacillales Lactobacillaceae

Genome size: 2  330  171 bp

Genomic GC% content: 38.47 %

Scanning electron microscopy (20 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Ornithine carbamoyltransferase (EC=2.1.3.3) 2295 Arginine biosynthesis
Arginine deiminase (EC=3.5.3.6) 594 Arginine biosynthesis
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 680 Glycolysis / Gluconeogenesis
Enolase (EC=4.2.1.11) 1191 Glycolysis / Gluconeogenesis
L-lactate dehydrogenase (EC=1.1.1.27) 789 Glycolysis / Gluconeogenesis
Pyruvate dehydrogenase (EC=1.2.4.1) 1598 Glycolysis / Gluconeogenesis
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase (EC=2.3.1.12) 688 Glycolysis / Gluconeogenesis
Alcohol dehydrogenase (EC=1.1.1.1) 536 Glycolysis / Gluconeogenesis
Glucose-6-phosphate 1-dehydrogenase (EC=1.1.1.49) 603 Pentose phosphate pathway
6-phosphogluconate dehydrogenase (EC=1.1.1.44) 785 Pentose phosphate pathway
D-arabino-3-hexulose 6-phosphate formaldehyde-lyase (EC=4.1.2.43) 4330 Pentose phosphate pathway
6-phospho-3-hexuloisomerase (EC=5.3.1.27) 690 Pentose phosphate pathway
Xylulose-5-phosphate phosphoketolase (EC=4.1.2.9) 937 Pentose phosphate pathway
Acetaldehyde dehydrogenase (EC=1.2.1.10) 1011 Phenylalanine metabolism
Maltose phosphorylase (EC=2.4.1.8) 689 Starch and sucrose metabolism

MS raw data: An71

culture medium: Wilkins-Chalgren Anaerobe Agar

16S rRNA sequence:

TGAGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATGCAAGTCGTACGCACTGGCCCAACTGATTGATGGTGCTTGCACCTGATTGACGATGGATTACCAGTGAGTGGCGGACGGGTGAGTAACACGTAGGTAACCTGCCCCGGAGCGGGGGATAACATTTGGAAACAGATGCTAATACCGCATAACAACAAAAGCCACATGGCTTTTGTTTGAAAGATGGCTTTGGCTATCACTCTGGGATGGACCTGCGGTGCATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGATGATGCATAGCCGAGTTGAGAGACTGATCGGCCACAATGGAACTGAGACACGGTCCATACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGATGGAGCAACACCGCGTGAGTGAAGAAGGGTTTCGGCTCGTAAAGCTCTGTTGTTGGAGAAGAACGTGCGTGAGAGTAACTGTTCACGCAGTGACGGTATCCAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTGCTTAGGTCTGATGTGAAAGCCTTCGGCTTAACCGAAGAAGTGCATCGGAAACCGGGCGACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGGAATGCGTAGATATATGGAAGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGATGAGTGCTAGGTGTTGGAGGGTTTCCGCCCTTCAGTGCCGGAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATCTTGCGCTAACCTTAGAGATAAGGCGTTCCCTTCGGGGACGCAATGACAGGTGGTGCATGGTCGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTACTAGTTGCCAGCATTAAGTTGGGCACTCTAGTGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGACGACGTCAGATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACGGTACAACGAGTCGCAAGCTCGCGAGAGTAAGCTAATCTCTTAAAGCCGTTCTCAGTTCGGACTGTAGGCTGCAACTCGCCTACACGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTTGTAACGCCCAAAGTCGGTGGCCTAACCTTTATGGAGGGAGCCGCCTAAGGCGGGACAGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGAGAACCTGCGGCTGGATCACCTCCTTT