Parasutterella secunda An562

Proteobacteria Betaproteobacteria Burkholderiales Sutterellaceae

Genome size: 1 979 860 bp

Genomic GC% content: 47.60 %

Scanning electron microscopy (11 000x magnification)

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Formate acetyltransferase (EC 2.3.1.54) 323 Pyruvate metabolism
Phosphoenolpyruvate synthase (EC 2.7.9.2) 208 Pyruvate metabolism
Glutamate racemase (EC 5.1.1.3) 133 Protein and amino acid degradation
Carboxypeptidase (EC 3.4.17.11) 141 Protein and amino acid degradation
Peptidyl-lysine N-acetyltransferase Pat (EC 2.3.1.-) 210 Protein and amino acid degradation
Isoaspartyl peptidase (EC 3.4.19.5) 195 Protein and amino acid degradation
Aspartate ammonia-lyase (EC 4.3.1.1) 628 Protein and amino acid degradation
L-asparaginase (EC 3.5.1.1) 150 Protein and amino acid degradation
Fumarate reductase flavoprotein subunit (EC 1.3.5.4) 607 Anaerobic respiration
ATP synthase (EC 7.1.2.2) 238 Oxidative phosphorylation
Phosphoribosylformylglycinamidine synthase (EC 6.3.5.3) 181 Nucleotide biosynthesis
Inosine-5′-monophosphate dehydrogenase (EC 1.1.1.205) 182 Nucleotide biosynthesis
Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) 138
Hydrogenase-1 (EC 1.12.99.6) 147
Periplasmic [Fe] hydrogenase (EC 1.12.7.2) 174

MS raw data: An562

16S rRNA sequence:

TAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATAGAGAGCTTGCTCTCTGATGGCGAGTGGCGAACGGGTGAGTAATACATCGGAACGTGTCCTGTTGTGGGGGATAACTACTCGAAAGAGTAGCTAATACCGCATAAGACCTACGGGTGAAAGCGGGGGACCTTCGGGCCTCGCGCAGTTGGAGCGGCCGATGACTGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCAGTAGCTGGTCTGAGAGGACGACCAGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGCGGGAAGAAGGCCTTCGGGTTGTAAACCGCTTTTGTTAGGAACGAAAAGGTTTCTGTGAACAACAGAGACTGCTGACGGTACCTAAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGGTAAGACAGGTGTGAAATCCCCGAGCTTAACTTGGGAACTGCACTTGTGACTGCTCAACTAGAGTATGTCAGAGGGAGGTGGAATTCCAAGTGTAGCAGTGAAATGCGTAGATATTTGGAAGAACACCGATGGCGAAGGCAGCCTCCTGGGATAATACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTTGGGCCGTATGGCTTAGTAACACAGCTAACGCGAGAAGTTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAAAACCTTACCTAGCCTTGACATGCCAGGAATCCTGATGAAAGTTGGGAGTGCCCGCAAGGGAATCTGGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCACTAGTTGCTACGCAAGAGCACTCTAGTGAAACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGGCTAGGGCCTCACACGTCATACAATGGTCGGAACAGAGGGCAGCGAAGCCGTGAGGTGGAGCCAATCCCAGAAAACCGATCGTAGTCCGGATTGCAGTCTGCAACTCGACTGCATGAAGTCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGGTTCACCAGAAGGCGTTTGCCTAACCTTCGGGAGGGCGGCGACCACGGTGGGTTTCATGACTGGGGTGAAGTCGTAACAAGGTAGCAGTACCGGAAGGTGCGGCTGGATCACCTCCTTT