Parasutterella secunda ET73

Proteobacteria Betaproteobacteria Burkholderiales Sutterellaceae

Genome size: 1 980 804 bp

Genomic GC% content: 46.60 %

Scanning electron microscopy (20 000x magnification)

 

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Formate acetyltransferase (EC=2.3.1.54) 550 Pyruvate metabolism
Phosphoenolpyruvate synthase (EC=2.7.9.2) 295 Pyruvate metabolism
Glutamate racemase (EC=5.1.1.3) 544 Protein and amino acid degradation
Carboxypeptidase (EC=3.4.17.11) 309 Protein and amino acid degradation
Peptidyl-lysine N-acetyltransferase (EC=2.3.1.-) 293 Protein and amino acid degradation
Isoaspartyl dipeptidase (EC=3.4.19.-) 232 Protein and amino acid degradation
Aspartate ammonia-lyase (EC=4.3.1.1) 2230 Protein and amino acid degradation
Fumarate hydratase class I, anaerobic (EC=4.2.1.2) 437 Citric cycle
Fumarate reductase iron-sulfur subunit (EC=1.3.5.1) 250 Anaerobic respiration
Fumarate reductase (EC=1.3.5.4) 1915 Anaerobic respiration
ATP synthase (EC=7.1.2.2) 635 ATP synthesis
ATP synthase (EC=7.1.2.2) 261 ATP synthesis
Phosphoribosylformylglycinamidine synthase (EC=6.3.5.3) 242 Purine metabolism
p-aminobenzoyl-glutamate hydrolase (EC=3.5.1.-) 222
Periplasmic [Fe] hydrogenase (EC=1.12.7.2) 270

MS raw data: ET73

culture medium: chocolate agar

16S rRNA sequence:

TGAACTTAAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGcTTTACACATGCAAGTCGAACGGCAGCATGGAGAGCTTGCTCTCTGATGGCGAGTGGCGAACGGGTGAGTAATACATCGGAACGTGTCCTGTTGTGGGGGATAACTACTCGAAAGAGTAGCTAATACCGCATAAGACCTAAGGGTGAAAGCGGGGGACCTTCGGGCCTCGCGCTATTGGAGCGGCCGATGACTGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCAGTAGCTGGTCTGAGAGGACGACCAGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGATCCAGCCATGCCGCGTGCGGGAAGAAGGCCTTCGGGTTGTAAACCGCTTTTGTTAGGAACGAAAAGGTATCTGTGAACAACAGGTATTGCTGACGGTACCTAAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGGTAAGACAGGTGTGAAATCCCCGAGCTTAACTTGGGAACTGCACTTGTGACTGCTCAACTAGAGTATGTCAGAGGGAGGTGGAATTCCAAGTGTAGCAGTGAAATGCGTAGATATTTGGAAGAACACCGATGGCGAAGGCAGCCTCCTGGGATAATACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTAGTTGTTGGGCCGCATGGCTTAGTAACACAGCTAACGCGAGAAGTTGACCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGATTAATTCGATGCAACGCGAAAAACCTTACCTAGCCTTGACATGCCAGGAATCCTGATGAAAGTTGGGAGTGCCCGCAAGGGAATCTGGACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCACTAGTTGCTACGCAAGAGCACTCTAGTGAAACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGGCTAGGGCCTCACACGTCATACAATGGTCGGAACAGAGGGCAGCGAAGCTGTGAAGTGGAGCCAATCCCAGAAAACCGATCGTAGTCCGGATTGCAGTCTGCAACTCGACTGCATGAAGTCGGAATCGCTAGTAATCGCGGATCAGCACGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTGGGGTTCACCAGAAGGCGTTTGCCTAACCTTCGGGAGGGCGGCGACCACGGTGGGTATCATGACTGGGGTGAAGTCGTAACAAGGTAGCAGTACCGGAAGGTGCGGCTGGATCACCTCCTTTCT