Phascolarctobacterium faecium ET69

Firmicutes Negativicutes Selenomonadales Acidaminococcaceae

Genomic GC% content: 1 885 819 bp

Obsah GC% v genomu: 45,70 %

Scanning electron microscopy (20 000x magnification)

TOP 15 in enzymes expressed during growth in BHI medium as determined by protein mass spectrometry

Enzyme PSM counts Pathway
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) 124 Glycolysis
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) 718 Succinate-Methylmalonate-Propionate conversion
Methylmalonyl-CoA mutase (EC 5.4.99.2) 514 Succinate-Methylmalonate-Propionate conversion
Ethylmalonyl-CoA/methylmalonyl-CoA epimerase (EC 5.1.99.-) 112 Succinate-Methylmalonate-Propionate conversion
Succinyl-CoA:coenzyme A transferase (EC 2.8.3.-) 334
Reverse rubrerythrin-1 (EC 1.11.1.1) 278
Glutamate dehydrogenase (EC 1.4.1.3) 249
Pyruvate synthase subunit PorC (EC 1.2.7.1) 225
Phosphoenolpyruvate synthase (EC 2.7.9.2) 158
Pyruvate:ferredoxin oxidoreductase (EC 1.2.7.1) 146
ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20) 148
Acetyl-CoA:oxalate CoA-transferase (EC 2.8.3.19) 139
2-oxoglutarate oxidoreductase subunit KorA (EC 1.2.7.3) 123
Cobalt-precorrin-8 methylmutase (EC 5.4.99.60) 112
Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) 106

MS raw data: ET69

culture medium: Schaedler agar + 20mM succinate

16S rRNA sequence:

GGTATATTGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAAATTGAGCTTCGGTTCAATTTTTAGTGGCGAACGGGTGAGTAACGCGTAGGCAACCTGCCCTTTAGACGGGGACAACATTCCGAAAGGGATGCTAATACCGGATGTGACGCTCCTATCGCATGACGGGAGCGTGAAAGATGGCCTCTACATGTAAGCTATCGCTAAAGGATGGGCCTGCGTCTGATTAGCTAGTTGGTAGTGTAACGGACAACCAAGGCGATGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGAGTGAAGAAGGTCTTCGGATTGTAAAGCTCTGTTGTACATGACGAATGTGCCGGTTGTGAATAATGGCTGGTAATGACGGTAGTGTACGAGGAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCCTATTAAGTCGGGCGTGAAAATGCGGGGCTCAACCCCGTATGGCGCCCGATACTGGTGGGCTTGAGTGCAGGAGAGGAAAGGGGAATTCCCAGTGTAGCGGTGAAATGCGTAGATATTGGGAGGAACACCAGTGGCGAAGGCGCCTTTCTGGACTGTGTCTGACGCTGAGATGCGAAAGCCAGGGGAGCGAACGGGATTAGATACCCCGGTAGTCCTGGCCGTAAACGATGGGTACTAGGTGTAGGAGGTATCGACCCCTTCTGTGCCGGAGTTAACGCAATAAGTACCCCGCCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAGGGCTTGACATTGAGCGCCATACTAAGAGATTAGTAGTTCCCTTCGGGGACGTGAAAACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTATCCTATGTTACCAGCACGTAAAGGTGGGGACTCATGGGAGACTGCCAGGGACAACCTGGAGGAAGGCGGGGATGACGTCAAGTCATCATGCCCCTTATGTCCTGGGCTACACACGTACTACAATGGTCGGCAACAGAGGGAAGCGAAGCCGCGAGGCAGAGCAAACCCCAGAAACCCGATCTCAGTTCGGATCGCAGGCTGCAACCCGCCTGCGTGAAGTCGGAATCGCTAGTAATCGCAGGTCAGCATACTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAAAGTTGGTAACACCCGAAGCCGGTGAGGTAACCTTTCAGGAGCCAGCCGTCTAAGGTGGGGCCGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTTCTA