Firmicutes | Clostridia | Clostridiales | Ruminococcaceae |
Velikost genomu: 3 474 625 bp
Obsah GC% v genomu: 53,61 %
Skenovací elektronová mikroskopie (zvětšení 11 000x)
TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií
Enzyme | PSM counts | Pathway |
6-phospho-beta-glucosidase (EC=3.2.1.86) | 214 | Glycolysis |
Pyrophosphate–fructose 6-phosphate 1-phosphotransferase (EC=2.7.1.90) | 267 | Glycolysis |
Fructose-bisphosphate aldolase (EC=4.1.2.13) | 234 | Glycolysis |
Triosephosphate isomerase (EC=5.3.1.1) | 473 | Glycolysis |
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) | 902 | Glycolysis |
2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC=5.4.2.12) | 180 | Glycolysis |
Enolase (EC=4.2.1.11) | 261 | Glycolysis |
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) | 579 | Glycolysis |
Pyruvate kinase (EC=2.7.1.40) | 314 | Glycolysis |
Acetyl-CoA acetyltransferase (EC=2.3.1.9) | 392 | Butyrate production |
Acyl-CoA dehydrogenase (EC=1.3.8.1) | 464 | Butyrate production |
Short-chain-enoyl-CoA hydratase (EC=4.2.1.150) | 312 | Butyrate production |
3-hydroxybutyryl-CoA dehydrogenase (EC=1.1.1.157) | 342 | Butyrate production |
4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA-Delta-isomerase (EC=4.2.1.120) | 163 | Butyrate production |
NADP-specific glutamate dehydrogenase (EC=1.4.1.4) | 182 | Arginine biosynthesis |
MS raw data: An179
kultivační médium: Wilkins-Chalgren Anaerobe Agar + CN
Sekvence 16S rRNA:
TTTAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGTTTGAGGAAATCCTTCGGGATGGAATCTTCCAACTTAGTGGCGGACGGGTGAGTAACGCGTGAGCAATCTGCCTTTCAGAGGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATAATGCATCGAATTCGCATGTTTTTGATGCCAAAGATTTTATCGCTGAAAGATGAGCTCGCGTCTGATTAGCTAGTTGGCGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGACGCAGCAACGCCGCGTGATTGATGAAGGTCTTCGGATTGTAAAAATCTTTAATCAGGGACGAAACAAATGACGGTACCTGAAGAATAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGCTTGTAAGTTGGAAGTGAAATCTCGGGGCTTAACCCCGAAACTGCTTTCAAAACTGCGAGTCTTGAGTGATGGAGAGGCAGGCGGAATTCCCAGTGTAGCGGTGAAATGCGTAGATATTGGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGACATTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGAGGTATTGACCCCTTCCGTGCCGGAGTTAACACAATAAGTATCCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCGATGACCGCTCTAGAGATAGGGCTTCTCTTCGGAGCATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTACGGTTAGTTGCTACGCAAGAGCACTCTAGCCGGACTGCCGTTGACAAAACGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCCCTTATGACTTGGGCTACACACGTACTACAATGGCAGTCATACAGAGGGAAGCAAAACCGCGAGGTGGAGCAAATCCCTAAAAGCTGTCCCAGTTCAGATTGCAGGCTGCAACTCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGCCGGTAATACCCGAAGTCCGTAGTCTAACCGCAAGGAGGACGCGGCCGAAGGTAGGACTGGTAATTAGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT