Firmicutes | Clostridia | Clostridiales | Lachnospiraceae |
Velikost genomu: 3 241 294 bp
Obsah GC% v obsahu: 44,78 %
Skenovací elektronová mikroskopie (zvětšení 10 000x)
TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií
Enzyme | PSM counts | Pathway |
ATP-dependent 6-phosphofructokinase (EC=2.7.1.11) | 105 | Glycolysis / Gluconeogenesis |
Triosephosphate isomerase (EC=5.3.1.1) | 82 | Glycolysis / Gluconeogenesis |
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) | 195 | Glycolysis / Gluconeogenesis |
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) | 278 | Glycolysis / Gluconeogenesis |
Pyruvate, phosphate dikinase (EC=2.7.9.1) | 163 | Glycolysis / Gluconeogenesis |
D-lactate dehydrogenase (EC=1.1.1.28) | 80 | Pyruvate metabolism |
Pyruvate carboxylase (EC=6.4.1.1) | 193 | Citrate cycle (TCA cycle) |
2-oxoglutarate oxidoreductase (EC=1.2.7.3) | 131 | Citrate cycle (TCA cycle) |
Acyl-CoA dehydrogenase, short-chain specific (EC=1.3.8.1) | 734 | Fatty acid degradation |
NAD-specific glutamate dehydrogenase (EC=1.4.1.2) | 405 | Arginine biosynthesis |
(R)-2-hydroxyisocaproyl-CoA dehydratase (EC=4.2.1.157) | 296 | |
2-aminoadipate transaminase (EC=2.6.1.39) | 160 | Lysine biosynthesis |
Phosphoserine aminotransferase (EC=2.6.1.52) | 85 | Glycine, serine and threonine metabolism |
NADH-dependent phenylglyoxylate dehydrogenase (EC=1.2.1.58) | 80 | Phenylalanine metabolism |
Proton-translocating ferredoxin:NAD(+) oxidoreductase (EC=7.1.1.-) | 164 |
MS raw data: An75
kultivační médium: Wilkins-Chalgren Anaerobe Agar
Sekvence 16S rRNA:
TAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATatTTagAATGAGAGCTTCGGCAGGATTTttaTCtATCTTAGTGGCGGACGGGTGAGTAACGTGTGGGCAACCTGCCCTGTACTGGGGAATAATCATTGGAAACGATGACTAATACCGCATGTGGTCCTCGGAAGGCATCTTcTGAGGAAGAAAGGATTTATTcGGTACAGGATGGGCCCGCATCTGATTAGCTAGTTGGTGAGATAACAGCCCACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCAACGCCGcGTGAAGGATGAAGGGTTTCGGCTCGTAAACTTCTATCAATAGGGAAGAAAgAAATGACGGTACCTAAATAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGCATGGTAAGCCAGATGTGAAAGCCTTGGGCTTAACCCGAGGATTGCATTTGGAACTATCAAGCTAGAGTACAGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAGTGGCGAAGGCGGCTTTCTGGACTGAAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTCGGGGAGGAATCCTCGGTGCCGCAGCTAACGCAATAAGCACTCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGGCTTGACATCCCGATGACCGctCTAGAGATAGagCTTCTCTTCGGAGCATCGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTCAGTAGCCATCATTcAGTTGGGCACTCTGGAGAGACTGCCGTGGATAACACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTTGGGCTACACACGTGCTACAATGGCTGGTAACAAAGTGACGCaAgACGGCGACGTTAAGCAAATCACAAAAACCCAGTCCCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGCATGTCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGAAGCACCCGAAGTCGGTGACCTAACCGTAAGGAAGGAGCCGCCGAAGGTGAAGCCAGTGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT