Firmicutes | Clostridia | Clostridiales | Ruminococcaceae |
Velikost genomu: 4 104 028 bp
Obsah GC% v obsahu: 53,46 %
Skenovací elektronová mikroskopie (zvětšení 20 000x)
TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií
Enzyme | PSM counts | Pathway |
Fructose-bisphosphate aldolase (EC=4.1.2.13) | 150 | Glycolysis/Gluconeogenesis |
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) | 355 | Glycolysis/Gluconeogenesis |
Phosphoglycerate kinase (EC=2.7.2.3) | 194 | Glycolysis/Gluconeogenesis |
Pyruvate, phosphate dikinase (EC=2.7.9.1) | 241 | Glycolysis/Gluconeogenesis |
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) | 324 | Glycolysis/Gluconeogenesis |
Aldehyde-alcohol dehydrogenase (EC=1.1.1.1) | 174 | Glycolysis/Gluconeogenesis |
Oxaloacetate decarboxylase (EC=7.2.4.2) | 184 | Pyruvate metabolism |
3-oxoacyl-[acyl-carrier-protein] reductase (EC=1.1.1.100) | 194 | Butyrate production |
Acyl-CoA dehydrogenase (EC=1.3.8.1) | 230 | Butyrate production |
3-hydroxybutyryl-CoA dehydrogenase (EC=1.1.1.157) | 157 | Butyrate production |
Acetyl-CoA acetyltransferase (EC=2.3.1.9) | 228 | Butyrate production |
Glycerol dehydrogenase (EC=1.1.1.6) | 267 | Glycerolipid metabolism |
4-hydroxy-tetrahydrodipicolinate synthase (EC=4.3.3.7) | 256 | Lysine biosynthesis |
4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC=1.17.7.4) | 170 | Terpenoid backbone biosynthesis |
Tryptophanase (EC=4.1.99.1) | 174 | Tryptophan metabolism |
MS raw data: An174
kultivační médium: Wilkins-Chalgren Anaerobe Agar + NEO
Sekvence 16S rRNA:
AAAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTACGTTTTGAAGTTTTCGGATGGATGAATGTAAGCTTAGTGGCGGACGGGTGAGTAACACGTGAGCAACCTGCCTTTCAGAGGGGGATAACAGCCGGAAACGGCTGCTAATACCGCATGATGTTGCGGGGGCACATGCCCCTGCAACCAAAGGAGCAATCCGCTGAAAGATGGGCTCGCGTCCGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGAAGACGGTCTTCGGATTGTAAACCTCTGTCTTTGGGGAAGAAAATGACGGTACCCAAAGAGGAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTAGAATGTTAAATCCATCGGCTCAACCGGTGGCTGCGTTCTAAACTGCCGTTCTTGAGTGAAGTAGAGGCAGGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGGCTTTAACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATTACTAGGTGTGGGGGGACTGACCCCTTCCGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCGGATGCATAGCCTAGAGATAGGTGAAGCCCTTCGGGGCATCCAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATTATTAGTTGCTACGCAAGAGCACTCTAATGAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTACTACAATGGCACTAAAACAGAGGGCGGCGACACCGCGAGGTGAAGCGAATCCCGAAAAAGTGTCTCAGTTCAGATTGCAGGCTGCAACCCGCCTGCATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTCGGTAACACCCGAAGCCAGTAGCCTAACCGCAAGGGGGGCGCTGTCGAAGGTGGGATTGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT