Firmicutes | Bacilli | Bacillales | Bacillaceae |
Velikost genomu: 4 243 165 bp
Obsah GC% v obsahu: 45,90 %
Skenovací elektronová mikroskopie (zvětšení 20 000x)
TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií
Enzyme | PSM counts | Pathway |
Carbamate kinase (EC 2.7.2.2) | 656 | Arginine biosynthesis |
Ornithine carbamoyltransferase (EC 2.1.1.3) | 505 | Arginine biosynthesis |
Arginine deiminase (EC 3.5.3.6) | 943 | Arginine biosynthesis |
Isocitrate dehydrogenase (EC 1.1.1.42) | 340 | Citrate cycle (TCA cycle) |
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) | 899 | Glycolysis/Gluconeogenesis |
Phosphoglycerate kinase (EC 2.7.2.3) | 407 | Glycolysis/Gluconeogenesis |
Phosphoglycerate mutase (EC 5.4.2.11) | 269 | Glycolysis/Gluconeogenesis |
Enolase (EC 4.2.1.11) | 366 | Glycolysis/Gluconeogenesis |
Pyruvate kinase (EC 2.7.1.40) | 612 | Glycolysis/Gluconeogenesis |
Alcohol dehydrogenase (EC 1.1.1.1) | 797 | Glycolysis/Gluconeogenesis |
Respiratory nitrate reductase (EC 1.7.1.1) | 431 | Nitrogen metabolism |
ATP synthase (EC 7.1.2.2) | 626 | Oxidative phosphorylation |
Pyruvate formate-lyase (EC 2.3 .1 .54) | 892 | Pyruvate metabolism |
Maltose-6ʼ-phosphate glucosidase (EC 3.2.1.22) | 665 | Starch and sucrose metabolism |
Beta-glucosidase (EC 3.2.1.21) | 450 | Starch and sucrose metabolism |
MS raw data:An441
kultivační médium: Wilkins-Chalgren Anaerobe Agar
Sekvence 16S rRNA:
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACCGACGGGAGCTTGCTCCCTTAGGTCAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGCTTGATTGAACCGCATGGTTCAATCATAAAAGGTGGCTTTTAGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAACTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAACCCTAGAGATAGGGCTTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGGCAGAACAAAGGGCAGCGAAGCCGCGAGGCTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT