Firmicutes | Clostridia | Clostridiales | Lachnospiraceae |
Velikost genomu: 4 340 741 bp
Obsah GC% v genomu: 44,56%
Skenovací elektronová mikroskopie (zvětšení 15 000x)
TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií
Enzyme | PSM counts | Pathway |
NADP-specific glutamate dehydrogenase (EC=1.4.1.4) | 61 | Arginine biosynthesis |
5-methyltetrahydrofolate:corrinoid/iron-sulfur protein co-methyltransferase (EC=2.1.1.258) | 62 | Reductive acetogenesis |
Carbon monoxide dehydrogenase/acetyl-CoA synthase (EC=2.3.1.169) | 85 | Reductive acetogenesis |
O-acetylserine sulfhydrylase (EC=2.5.1.47) | 66 | Cysteine and methionine metabolism |
Fructose-bisphosphate aldolase (EC=4.1.2.13) | 57 | Glycolysis / Gluconeogenesis |
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) | 63 | Glycolysis / Gluconeogenesis |
Phosphoenolpyruvate carboxykinase (ATP) (EC=4.1.1.49) | 124 | Glycolysis / Gluconeogenesis |
Pyruvate, phosphate dikinase (EC=2.7.9.1) | 197 | Glycolysis / Gluconeogenesis |
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) | 206 | Glycolysis / Gluconeogenesis |
Aldehyde-alcohol dehydrogenase (EC=1.1.1.1) | 220 | Glycolysis / Gluconeogenesis |
Phosphate propanoyltransferase (EC=2.3.1.222) | 50 | Propanoate metabolism |
NAD-dependent malic enzyme (EC=1.1.1.38) | 52 | Pyruvate metabolism |
Oxaloacetate decarboxylase (EC=7.2.4.2) | 51 | Pyruvate metabolism |
Propionyl-CoA carboxylase (EC=6.4.1.3) | 102 | Valine, leucine and isoleucine degradation |
Carbon monoxide dehydrogenase (EC=1.2.7.4) | 63 | Reductive acetogenesis |
MS raw data: An81
kultivační médium: Wilkins-Chalgren Anaerobe Agar + NEO
Sekvence 16S rRNA:
ATCAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCCTGCTTTTGATTCTTTCGGGATGAAGAAGCTTGCGACTGAGCGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCTCACACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGGCcGCATGGCCCGGTGTGAAAAGCTCCGGCGGTGTGAGATGGACCTGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGCGAAGAAGTATCTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGAAGCAAGTCTGGAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTTTTTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGGAGCAAAGCTCTTCGGTGCCGCAGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGTTCcTTAACCGGAACTTTCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTCCTTAGTAGCCAGCAGTAAGATGGGCACTCTAGGGAGACAGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGCCTGCGAGGGGGAGCGAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCGGGACGGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT