Blautia producta An81

Firmicutes Clostridia Clostridiales Lachnospiraceae

Velikost genomu: 4 340 741 bp

Obsah GC% v genomu: 44,56%

Skenovací elektronová mikroskopie (zvětšení 15 000x)

 

TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií

Enzyme PSM counts Pathway
NADP-specific glutamate dehydrogenase (EC=1.4.1.4) 61 Arginine biosynthesis
5-methyltetrahydrofolate:corrinoid/iron-sulfur protein co-methyltransferase (EC=2.1.1.258) 62 Reductive acetogenesis
Carbon monoxide dehydrogenase/acetyl-CoA synthase (EC=2.3.1.169) 85 Reductive acetogenesis
O-acetylserine sulfhydrylase (EC=2.5.1.47) 66 Cysteine and methionine metabolism
Fructose-bisphosphate aldolase (EC=4.1.2.13) 57 Glycolysis / Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 63 Glycolysis / Gluconeogenesis
Phosphoenolpyruvate carboxykinase (ATP) (EC=4.1.1.49) 124 Glycolysis / Gluconeogenesis
Pyruvate, phosphate dikinase (EC=2.7.9.1) 197 Glycolysis / Gluconeogenesis
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) 206 Glycolysis / Gluconeogenesis
Aldehyde-alcohol dehydrogenase (EC=1.1.1.1) 220 Glycolysis / Gluconeogenesis
Phosphate propanoyltransferase (EC=2.3.1.222) 50 Propanoate metabolism
NAD-dependent malic enzyme (EC=1.1.1.38) 52 Pyruvate metabolism
Oxaloacetate decarboxylase (EC=7.2.4.2) 51 Pyruvate metabolism
Propionyl-CoA carboxylase (EC=6.4.1.3) 102 Valine, leucine and isoleucine degradation
Carbon monoxide dehydrogenase (EC=1.2.7.4) 63 Reductive acetogenesis

MS raw data: An81

kultivační médium: Wilkins-Chalgren Anaerobe Agar + NEO

Sekvence 16S rRNA:

ATCAGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCCTGCTTTTGATTCTTTCGGGATGAAGAAGCTTGCGACTGAGCGGCGGACGGGTGAGTAACACGTAGGCAACCTGCCTCACACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGCGCACAGGGCcGCATGGCCCGGTGTGAAAAGCTCCGGCGGTGTGAGATGGACCTGCGTCTGATTAGCCAGTTGGCGGGGTAACGGCCCACCAAAGCGACGATCAGTAGCCGGCCTGAGAGGGTGGACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGCGAAGAAGTATCTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGAAGCAAGTCTGGAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTTTTTCTTGAGTGCCGGAGAGGTAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTCGGGGAGCAAAGCTCTTCGGTGCCGCAGCAAACGCAGTAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAAGTCTTGACATCCCTCTGACCGTTCcTTAACCGGAACTTTCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCCTGTCCTTAGTAGCCAGCAGTAAGATGGGCACTCTAGGGAGACAGCCGGGGATAACCCGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCCCTTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCGAGCCTGCGAGGGGGAGCGAATCCCAAAAATAACGTCCCAGTTCGGACTGCAGTCTGCAACTCGACTGCACGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGCAAGGAGGGAGCTGCCGAAGGCGGGACGGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT