Firmicutes | Clostridia | Clostridiales | Lachnospiraceae |
Velikost genomu: 3 190 622 bp
Obsah GC% v genomu: 46,76 %
Skenovací elektronová mikroskopie (zvětšení 20 000x)
TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií
Enzyme | PSM counts | Pathway |
L-aspartate oxidase (EC 1.4.3.16) | 345 | Alanine, aspartate and glutamate metabolism |
Glutamate synthase [NADPH] (EC 1.4.1.13) | 480 | Alanine, aspartate and glutamate metabolism |
Glutamate dehydrogenase (EC 1.4.1.4) | 280 | Alanine, aspartate and glutamate metabolism |
Glutamine synthetase type III (EC 6.3.1.2) | 461 | Alanine, aspartate and glutamate metabolism |
Fructose-bisphosphate aldolase class II (EC 4.1.2.13) | 291 | Glycolysis/Gluconeogenesis |
Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) | 1081 | Glycolysis/Gluconeogenesis |
Phosphoglycerate kinase (EC 2.7.2.3) | 291 | Glycolysis/Gluconeogenesis |
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) | 733 | Glycolysis/Gluconeogenesis |
Pyruvate,phosphate dikinase (EC 2.7.9.1) | 766 | Glycolysis/Gluconeogenesis |
Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-) | 752 | Glycolysis/Gluconeogenesis |
Alcohol dehydrogenase (EC 1.1.1.1) | 245 | Glycolysis/Gluconeogenesis |
CoB-CoM heterodisulfide reductase (EC 1.8.98.1) | 259 | Methane metabolism |
Pyruvate formate-lyase (EC 2.3.1.54) | 273 | Pyruvate metabolism |
Malic enzyme (EC 1.1.1.40) | 262 | Pyruvate metabolism |
Ketol-acid reductoisomerase (EC 1.1.1.86) | 248 | Valine, leucine and isoleucine biosynthesis |
MS raw data: An298
kultivační médium: Wilkins-Chalgren Anaerobe Agar
Sekvence 16S rRNA:
CGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTCTGCTTTGATTCTTCGGATGAAGAGCATTGTGACTGAGCGGCGGACGGGTGAGTAACGCGTGGGTAACCTGCCTCATACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGCACCGCATGGTGCAGGGGTAAAAACTCCGGTGGTATGAGATGGACCCGCGTCTGATTAGCTAGTTGGTAAGGTAACGGCTTACCAAGGCGACGATCAGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAGCGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGAAACTGACGGTACCTGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAAGTGAAAGCCCGGGGCTCAACCCCGGGACTGCTTTGGAAACTGTTCATCTAGAGTGCTGGAGAGGTAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTACTGGACAGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTGCTAGGTGTCGGGTGGCAAAGCCATTCGGTGCCGCAGCTAACGCAATAAGCAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGCCCTTGACATCCGAATGACCGGTGAGTAATGTCACCTTTCCTTCGGGACATTCGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTAGTAGCCAGCAGTCCGGCTGGGCACTCTAGAGAGACTGCCAGGGATAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGGGCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAGGCAGAGCCGCGAGGCCGAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTCAGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGCGGGACCGATAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT