Lachnoclostridium phocaeense An118

Firmicutes Clostridia Clostridiales Lachnospiraceae

Velikost genomu: 3  480  620 bp

Obsah GC% v genomu: 52,25%

Skenovací elektronová mikroskopie (zvětšení 20 000x)

 

 

TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií

Enzyme PSM counts Pathway
Alanine dehydrogenase 2 (EC=1.4.1.1) 133 Alanine, aspartate and glutamate metabolism
Glutamine–fructose-6-phosphate aminotransferase [isomerizing] (EC=2.6.1.16) 107 Alanine, aspartate and glutamate metabolism
NADP-specific glutamate dehydrogenase (EC=1.4.1.4) 165 Arginine biosynthesis
Fructose-bisphosphate aldolase (EC=4.1.2.13) 132 Glycolysis / Gluconeogenesis
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) 241 Glycolysis / Gluconeogenesis
Phosphoenolpyruvate carboxykinase (EC=4.1.1.49) 188 Glycolysis / Gluconeogenesis
Pyruvate, phosphate dikinase (EC=2.7.9.1) 452 Glycolysis / Gluconeogenesis
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) 709 Glycolysis / Gluconeogenesis
Aldehyde-alcohol dehydrogenase (EC=1.1.1.2) 1060 Glycolysis / Gluconeogenesis
Formate acetyltransferase (EC=2.3.1.54) 240 Pyruvate metabolism
Oxaloacetate decarboxylase (EC=7.2.4.2) 227 Pyruvate metabolism
Maltodextrin phosphorylase (EC=2.4.1.1) 134 Starch and sucrose metabolism
3-isopropylmalate dehydratase (EC=4.2.1.33) 129 Valine, leucine and isoleucine biosynthesis
Propionyl-CoA carboxylase (EC=6.4.1.3) 350 Valine, leucine and isoleucine degradation
(R)-phenyllactyl-CoA dehydratase (EC=4.2.1.-) 108

MS raw data: An118

kultivační médium: Wilkins-Chalgren Anaerobe Agar

Sekvence 16S rRNA:

CGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTACTTTTGATTTCTTCGGAATGAGGAGGTCTGTGACTTAGCGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTCACACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGCACCGCATGGTGCAGGGGTAAAAACTCCGGTGGTGTGAGATGGGCCCGCGTCTGATTAGGTAGTTGGTGCGGTAACGGCGCACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGCGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGGAGTGAAAACCCGGGGCTCAACCCCGGGATTGCTTTGGAAACTGTGTAGCTGGAGTACCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGTGAGCAAAGCTCATCGGTGCCGCAGCCAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCCTTTGACCGGCAAGTAATGTTGCCTTTCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTACCTTCAGTAACCAGCACGTAATGGTGGGGACTCTGGAGGGACTGCCAGGGACAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAGGCTGTGAAGCGGAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGCGGGACCGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT