Firmicutes | Clostridia | Clostridiales | Lachnospiraceae |
Velikost genomu: 3 480 620 bp
Obsah GC% v genomu: 52,25%
Skenovací elektronová mikroskopie (zvětšení 20 000x)
TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií
Enzyme | PSM counts | Pathway |
Alanine dehydrogenase 2 (EC=1.4.1.1) | 133 | Alanine, aspartate and glutamate metabolism |
Glutamine–fructose-6-phosphate aminotransferase [isomerizing] (EC=2.6.1.16) | 107 | Alanine, aspartate and glutamate metabolism |
NADP-specific glutamate dehydrogenase (EC=1.4.1.4) | 165 | Arginine biosynthesis |
Fructose-bisphosphate aldolase (EC=4.1.2.13) | 132 | Glycolysis / Gluconeogenesis |
Glyceraldehyde-3-phosphate dehydrogenase (EC=1.2.1.12) | 241 | Glycolysis / Gluconeogenesis |
Phosphoenolpyruvate carboxykinase (EC=4.1.1.49) | 188 | Glycolysis / Gluconeogenesis |
Pyruvate, phosphate dikinase (EC=2.7.9.1) | 452 | Glycolysis / Gluconeogenesis |
Pyruvate:ferredoxin oxidoreductase (EC=1.2.7.1) | 709 | Glycolysis / Gluconeogenesis |
Aldehyde-alcohol dehydrogenase (EC=1.1.1.2) | 1060 | Glycolysis / Gluconeogenesis |
Formate acetyltransferase (EC=2.3.1.54) | 240 | Pyruvate metabolism |
Oxaloacetate decarboxylase (EC=7.2.4.2) | 227 | Pyruvate metabolism |
Maltodextrin phosphorylase (EC=2.4.1.1) | 134 | Starch and sucrose metabolism |
3-isopropylmalate dehydratase (EC=4.2.1.33) | 129 | Valine, leucine and isoleucine biosynthesis |
Propionyl-CoA carboxylase (EC=6.4.1.3) | 350 | Valine, leucine and isoleucine degradation |
(R)-phenyllactyl-CoA dehydratase (EC=4.2.1.-) | 108 |
MS raw data: An118
kultivační médium: Wilkins-Chalgren Anaerobe Agar
Sekvence 16S rRNA:
CGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTACTTTTGATTTCTTCGGAATGAGGAGGTCTGTGACTTAGCGGCGGACGGGTGAGTAACGCGTGGGCAACCTGCCTCACACAGGGGGATAACAGTTAGAAATGACTGCTAATACCGCATAAGACCACAGCACCGCATGGTGCAGGGGTAAAAACTCCGGTGGTGTGAGATGGGCCCGCGTCTGATTAGGTAGTTGGTGCGGTAACGGCGCACCAAGCCGACGATCAGTAGCCGACCTGAGAGGGCGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGAAGGATGAAGTATTTCGGTATGTAAACTTCTATCAGCAGGGAAGAAAATGACGGTACCTGACTAAGAAGCCCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGGAGTGAAAACCCGGGGCTCAACCCCGGGATTGCTTTGGAAACTGTGTAGCTGGAGTACCGGAGAGGCAAGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAGGCGGCTTGCTGGACGGTAACTGACGTTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGACTACTAGGTGTCGGTGAGCAAAGCTCATCGGTGCCGCAGCCAACGCAATAAGTAGTCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCTGGTCTTGACATCCCTTTGACCGGCAAGTAATGTTGCCTTTCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTACCTTCAGTAACCAGCACGTAATGGTGGGGACTCTGGAGGGACTGCCAGGGACAACCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCAGGGCTACACACGTGCTACAATGGCGTAAACAAAGAGAAGCGAGGCTGTGAAGCGGAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCGCGGATCAGAATGCCGCGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAACGCCCGAAGTCAGTGACCCAACCGTAAGGAGGGAGCTGCCGAAGGCGGGACCGGTAACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT