Megasphaera stantonii An286

Firmicutes Negativicutes Selenomonadales Veillonellaceae

Velikost genomu: 2 396 433 bp

Obsah GC% v obsahu: 53,29 %

Skenovací elektronová mikroskopie (zvětšení 20 000x)

TOP 15 enzymů exprimovaných během růstu v BHI mediu stanovené proteinovou hmotnostní spektrometrií

Enzyme PSM counts Pathway
NADP-specific glutamate dehydrogenase (EC 1.4.1.4) 669
2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3) 748
2-oxoglutarate oxidoreductase, gamma subunit (EC 1.2.7.3) 416
2-oxoglutarate oxidoreductase, beta subunit (EC 1.2.7.3) 369
Acetyl-CoA acetyltransferase (EC 2.3.1.9) 2773 Butyrate production
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) 667 Butyrate production
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) 408 Butyrate production
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) 1494 Butyrate production
Butyryl-CoA dehydrogenase (EC 1.3.99.2) 3370 Butyrate production
4-hydroxybutyrate coenzyme A transferase (EC:2.8.3.-) 770 Butyrate production
Fructose-bisphosphate aldolase class II (EC 4.1.2.13) 244 Glycolysis / Gluconeogenesis
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) 239 Glycolysis / Gluconeogenesis
Inosine-5′-monophosphate dehydrogenase (EC 1.1.1.205) 260 Purine biosynthesis
L-lactate dehydrogenase (EC 1.1.1.27) 729 Pyruvate metabolism
Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-) 632 Pyruvate metabolism

MS raw data: An286

Sekvence 16S rRNA:

GTTGGAGGGGTAACGGCCCACCAAGGCGACGATCAGTAGCCGGTCTGAGAGGATGAACGGCCACATTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGACGGAGCAACGCCGCGTGAGCGAAGACGGCCTTCGGGTTGTAAAGCTCTGTTATACGGGACGAACGGCTAGTGTGCCAATACCACATTAGAATGACGGTACCGTAAGAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCAGGCGGTTTCATAAGTCTGTCTTAAAAGTGCGGGGCTTAACCCCGTGAGGGGACGGAAACTGTGAGACTGGAGTGTCGGAGAGGAAAGCGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAAAGCGGCTTTCTGGACGACAACTGACGCTGAGGCGCGAAAGCCAGGGGAGCGAACGGGATTAGATACCCCGGTAGTCCTGGCCGTAAACGATGGATACTAGGTGTAGGGGGTATCGACCCCTCCTGTGCCGGAGTTAACGCAATAAGTATCCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGCCTTGACATT